Experimental: Wetlab Team
Clonebots Assembly
Project Leads: Pritika Acharya, Shing Gao
Members: Anika Hauser, Anish Iyyavoo, Arvind Swamynathan, Cherry Woo, Dimple Amitha Garuadapuri, Ryan Idnani
Traditional plasmid assembly methods (e.g. Golden Gate, Gibson) are performed in vitro, requiring many intermediate steps. Our team utilizes a CRISPR system to construct DNA parts in vivo, resulting in a faster and more efficient way to perform DNA assembly. We are presently optimizing double transformation of indicator and editor plasmids, as well as testing conjugation of pOX28 plasmids.